Description
Features
- More efficient ECM than human-derived fibrin (Sieminski & Gooch 2004)
- Slower gel degradation in vitro and in vivo
- Excellent selection & proliferation of stem cells (Zhu et al. 2017)
- Superior performance for neurite & neuronal cell growth (Ju et al. 2007)
- Provides non-linear elasticity & soft compliance, improved cell migration, growth and differentiation
- Higher biocompatibility
- Allows easier optimization of cell culture conditions
- Non-carcinogenic, low toxicity and immunogenicity
- Intended for research use only
Application
- Non-mammalian extracellular matrix
- Regenerative medicine (Ju et al. 2007, Sharp et al. 2012, Arulmoli et al. 2016)
- Tumorigenic cell selection & growth (Liu et al. 2012)
- Stem cell selection & proliferation (Zhu et al. 2017)
Formulation
- Lyophilized, 100 mg
- > 90% clottable protein
- > 20 mg/ml protein
- Rheology – Shear Modulus (2% strain, 1 Hz, 1200 sec) > 1000 Pa
- Endotoxin < 10.0 EU/ml
- Sterility tested
- Custom unit size, excipients, non-lyophilized preparations available
Storage: Below -60oC
Unit of Sale: > 100 mg glass vial
Shelf Life: 3 years from manufacture
References
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Zhu P, Zhou Y, Wu F, Hong Y, Wang X, Shekhawat G, et al. (2017) Selective expansion of skeletal muscle stem cells from bulk muscle cells in soft three-dimensional fibrin gel. Stem Cells Translat Med 6:1412-1423. http://dx.doi.org/10.1002/sctm.16-0427
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Liu J, Tan Y, Zhang H, Zhang Y, Xu P, Chen J, et al. (2012) Soft fibrin gels promote selection and growth of tumorigenic cells. Nature Mater 11: 734–741. https://pubmed.ncbi.nlm.nih.gov/22751180/
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Ju YE, Janmey PA, McCormick ME, Sawyer ES, Flanagan LA (2007) Enhanced neurite growth from mammalian neurons in three-dimensional salmon fibrin gels. Biomaterials 28: 2097-2108. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1991290/
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Sieminski A & Gooch KJ (2004) Salmon fibrin supports an increased number of sprouts and decreased degradation while maintaining sprout length relative to human fibrin in an in vitro angiogenesis model. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3437931/
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Arulmoli J, Wright HJ, Phan DTT, Sheth U, Que RA, Botten GA, et al. (2017) Combination scaffolds of salmon fibrin, hyaluronic acid, and laminin for human neural stem cell and vascular tissue engineering. Acta Biomater. 43: 122-138. https://pubmed.ncbi.nlm.nih.gov/27475528/
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Sharp KG, Dickson AR, Marchenko SA, Yee KM, Emery PN, Laidmae I, et al. (2012) Salmon fibrin treatment of spinal cord injury promotes functional recovery and density of serotonergic innervation. Exp Neurol 235(1): 345-356. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3437931/
