Description
Features
- Non-mammalian
- Reduced non-specific activity
- Increased signal to noise ratio, high sensitivity
- Minimal cross-species interaction in mammalian-based assays
- Intended for research use only
Application
Membrane blocking as well as antibody dilution
Compatible with a wide range of applications:
- Western blotting (Puls et al. 2017, Rihn et al. 2021)
- Immunoblotting w/infrared detection (Jensen et al. 2018)
- ELISA (Joyner et al. 2019, Gordon et al. 2022, Hägglund et al. 2022)
- IHC (Pinkert-Leetsch et al. 2023, Tokarska et al. 2023)
- Immunofluorescence (Davis et al. 2021)
Formulation
Available in PBS or TRIS
0.22 mm filtered, pH 7.4 (+/-0.1), protein 0.8 mg/ml
Ready to use; can be diluted up to 10-fold, as needed
Custom preparations available
Storage: 4-8oC
Unit of Sale: 250 ml and 500 ml bottle
Shelf Life: One year from manufacture
References
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Davis MM, Brock AM, DeHart TG, Boribong BP, Lee K, McClune ME, et al. (2021). The peptidoglycan-associated protein NapA plays an important role in the envelope integrity and in the pathogenesis of the lyme disease spirochete. PLoS Pathog 17(5): e1009546.
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Rihn SJ, Merits A, Bakshi S, Turnbull ML, Wickenhagen A, Alexander AJT, et al. (2021). A plasmid DNA-launched SARS-CoV-2 reverse genetics system and coronavirus toolkit for COVID19 research. PLoS Biol 19(2): e3001091.
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Gordon L, Mabbott N, Wells J, Kulik L, Juleff N, Charleston B, et al. (2022). Foot-and mouth disease virus localization on follicular dendritic cells and sustained induction of neutralizing antibodies is dependent on binding to complement receptors (CR2/CR1). PLoS Pathog 18(5): e1009942.
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Joyner CJ, Brito CFA, Saney CL, Joice Cordy R, Smith ML, Lapp SA, et al. (2019). Humoral immunity prevents clinical malaria during Plasmodium relapses without eliminating gametocytes. PLoS Pathog 15(9): e1007974.
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Hägglund S, Näslund K, Svensson A, Lefverman C, Enül H, Pascal L, et al. (2022). Longitudinal study of the immune response and memory following natural bovine respiratory syncytial virus infections in cattle of different age. PLoS ONE 17(9): e0274332.
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Jensen IJ, Winborn CS, Fosdick MG, Shao P, Tremblay MM, Shan Q, et al. (2018). Polymicrobial sepsis influences NK-cell-mediated immunity by diminishing NK-cell-intrinsic receptor-mediated effector responses to viral ligands or infections. PLoS Pathog 14(10): e1007405.
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Puls TJ, Tan X, Whittington CF, Voytik-Harbin SL (2017). 3D collagen fibrillar microstructure guides pancreatic cancer cell phenotype and serves as a critical design parameter for phenotypic models of EMT. PLoS ONE 12(11): e0188870.
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Tokarska N, Naniong JMA, Johnston JM, Salapa HE, Muir GD, Levin MC, Popescu BF, Verge VMK (2023). Acute intermittent hypoxia alters disease course and promotes CNS repair including resolution of inflammation and remyelination in the experimental autoimmune encephalomyelitis model of MS. GLIA 71(8): p. 2045-2066.
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Pinkert-Leetsch D, Frohn J, Ströbel P, Alves F, Salditt T, Missbach-Guentner J (2023). Three-dimensional analysis of human pancreatic cancer specimens by phase-contrast based X-ray tomography – the next dimension of diagnosis. Cancer Imaging 23, 43 (2023).
Sea Block® is a registered trademark of Salmonics LLC.
